The Eppendorf Video Guide to Perfecting Your Pipetting Technique

Pipettes are among the most fundamental instruments used in modern laboratories for a plethora of applications; from PCR and cell culture to next generation sequencing preparation, chemical titrations, and drug discovery. So, let’s talk about possible choices and pipetting technique - using your pipettes correctly and efficiently, taking care of them properly, and choosing the best one for your application is crucial to getting the most out of your experiments. Whether you’re a pipetting novice wanting to go pro or simply want to brush up on some skills, this new video series from pipetting experts at Eppendorf has got you covered! We’ll begin by taking you back to the basics.

Episode 1: How to Pipette in 5 Simple Steps
In the first episode of the Pipetting Tutorials with Eppendorf series, you’ll be going back to the fundamental principles underlying good pipetting technique when using mechanical air-cushion pipettes (such as the Eppendorf Research® plus or Eppendorf Reference® 2) . With five simple and easy to follow steps that can be applied to nearly all pipetting applications, this video is packed full of useful tips and tricks – like pre-wetting your pipette tip!

Episode 2: How to Fill Plates Faster
If you’ve mastered the single-channel pipette but your thumbs are getting tired filling 96-well plates, fear not, the multi-channel pipette is here to make your work faster and more ergonomic. Discover how to choose the best multi-channel pipette and some helpful hints on how to use it effectively to boost productivity in your lab.

Episode 3: How to Increase Pipetting Efficiency
It’s no secret that Eppendorf Xplorer® electronic pipettes are a simple solution to boosting efficiency. But this isn’t their only benefit. From superior ergonomics to better reproducibility, electronic pipettes are transforming the modern laboratory. In this video, you’ll discover all the reasons why it’s time to make the move to electronic pipetting.

Episode 4: What’s Happening Inside Your Pipette?Have you ever thought about what’s going on inside your sleek, ergonomically designed Eppendorf pipette? Of course you have! This episode will walk you through the differences between the two major operating principles: positive displacement (like the Multipette®/ Combitips® system) and air-cushion systems. You may be wondering when to choose which system – find out the answer in this video!

Episode 5: How to Pipette Challenging Liquids
From viscous and volatile to oily and foaming, some liquids just won’t behave when it comes to pipetting them. But arm yourself with a few tips and tricks from this video and the right tools and techniques and you’ll be able to master these challenging liquids with ease.

Episode 6: How to Take Care of Your Pipettes
We know that pipettes are one of the most used and most important instruments in the lab so taking care of them should be top of your priority list. While sending it off to a professional maintenance and calibration provider like epServices at least once a year is a must, we’ll cover the things you can do yourself to keep your pipettes in tip top condition.

Episode 7: How to Choose the Right Pipette Tips for Your Experiment
Pipette tips are the Robin to the pipette’s Batman. The Watson to its Sherlock. The Shaggy to its Scooby-Doo. Essentially, both parts need to be right for the job to ensure the best results. In the penultimate part to this video series, we’ll show you all the different types of pipette and dispenser tips – and, most importantly, how to choose the right one for your application.

Episode 8: Liquid Handling with Bottle-Top Dispensers
If you’re not sure about the difference between a bottle-top dispenser (like the Varispenser® 2/2x ) and a bottle-top burette (such as the Eppendorf Top Buret® ), this video was made for you. And even if you already know the difference, this video is crammed with tips on how best to operate bottle-top dispensers. Check it out!

Having good pipetting technique is fundamental in most laboratories and with the information in our Pipetting Tutorials with Eppendorf video series, you could drastically improve your accuracy and precision. So, if you are ready to perfect your pipetting technique, check out our video series now.